We calculated metagenes as a surrogate for all genes contained within a particular cluster and visualized the relative expression in relation to time to metastasis with principal component analysis. 我们以特定基因族里的转位基因为代表用主成分分析法计算其与肿瘤转移时间的表达。
Biomimetic affinity purification of recombinant human lactoferrin from the milk of transgenic cows Relative expression of lactoferrin in the mammary gland of mice under immune state 转基因牛乳中重组人乳铁蛋白的仿生亲和纯化免疫小鼠乳腺乳铁蛋白基因表达变化研究
Relationship between the Contents of Soybean Isoflavanones and Relative Expression Quantity of PAL Gene 大豆叶片异黄酮含量与PAL基因相对表达量的关系
Real-time PCR detection of relative expression of sBD-1 mRNA of epithelial cell of fallopian tubes in Mongolian sheep 蒙古绵羊输卵管上皮细胞β-防御素mRNA相对表达水平的实时荧光定量PCR检测
Relative expression levels of HSP90 gene increased with the increase of temperature. 幼虫体内HSP90表达量随着温度升高呈增加的趋势。
The expression patterns of IGF-I R, IGF-II, IGF-IIR and InR in ovary, oviduct and uterus were similar, the relative expression in oviduct was higher than that in ovary and uterus. IGF-ⅠR、IGF-Ⅱ、IGF-ⅡR和InR在卵巢、输卵管和子宫中的表达规律相似,都是在输卵管中的相对表达量高于卵巢和子宫。
Ug mRNA was reverse transcripted to cDNA and hUAT and GAPDH were amplified by real-time fluorescent quantitative PCR. Then relative expression quantity of hUAT mRNA was analyzed. 取1ugRNh逆转录为cDNA,荧光定量PCR共扩增hUAT和内参照GAPDH目的片段,分析计算hUATmRNA相对表达量。
The relative expression of estrogen receptor and low-density lipoprotein receptor mRNA in the heart and liver of model rabbits were 0.29 ± 0.03, 0.27 ± 0.06 and 0.04 ± 0.01, 0.17 ± 0.02 respectively. 模型组,雌激素受体和低密度脂蛋白受体在心脏、肝脏内的相对表达量分别为0.29±0.03、0.27±0.06和0.04±0.01、0.17±0.02。
The relative expression amount in the spleen cells did not change obviously. 在移植过程中,脾细胞MIF相对表达量无明显变化。
Study on the Construction of hTR Relative Expression in Cells, and Study on the Detection of Telomerase Activity hTR基因在细胞中的表达及检测端粒酶活性方法的研究
Conclusions: The level of CD44 did not predict relative expression of the virus. 结论:CD44分子的表达水平不能反映体内病毒的拷贝数。
The ratio of integrated optical density of bFGF mRNA expression to that of GAPDH mRNA expression ( b/ G) was calculated to represent the relative expression amount of bFGF mRNA in the tissues. 计算BfgfmRNA与GAPDHmRNA表达积分光密度值的比值(b/G),以反映组织中BfgfmRNA的相对表达量。
And then, according to H.Rouse's equation of vertical distribution of suspended sediment, three kinds of relative expression of Pi, namely, are given. 根据H.Rouse含沙量分布公式,导出了相应的Pi/Pai、Pi/PipjQ等3种形式的悬沙级配沿垂线相对分布表达式;
We determinated the relative expression of leptin-mRNA in placenta by semi-quantitative nested-RT-PCR and analysised its relationships with pregnant related factors such as leptin in maternal blood and cord blood, placental weight and fetal birth weight. 瘦素mRNA的相对表达量采用半定量巢式逆转录聚合酶链反应(NestedRT-PCR)技术,分析其与母血瘦素、脐血瘦素、胎盘重量、胎儿出生体重等妊娠因素的关系。
The relative expression of receptor activator of nuclear factor-kappa B ligand and osteoprotegerin mRNA in bone tissue in ovariectomized mice 破骨细胞分化因子和护骨素mRNA在OVX小鼠骨组织的相对表达
Objective To study the relative expression levels of galectin-3 mRNA in benign and malignant thyroid tumor tissue as well as thyroid normal tissue. 目的探讨galectin3mRNA在良性和恶性甲状腺肿瘤组织中的表达情况。
Meanwhile, the content of anthocyanins had significant consistency with the relative expression of VcCHS, and they both showed the highest transcript abundance in the skins. 同时,发现从绿果期到果实成熟期,越橘花色素苷的蓄积量与VcCHS基因的表达量呈现一致性,二者都是在果皮组织中的含量达到最高。
The results demonstrated that the two genes were expressed in lateral roots, stems, leaves and floral and the relative expression level of stems were the highest. 两个关键酶基因在茎、叶、花、侧根中均有表达,其中茎中的相对表达量最高,侧根中最低。
Added a certain amount of STZ into medium of islet cells, and determined the relative expression of islet cell CSD gene in STZ and control groups by way of Semi-Quantitative PCR. 4. 向胰岛细胞的培养基中加入一定量STZ,并用半定量PCR的方法测定STZ组和对照组胰岛细胞CSD基因的相对表达量。
On the basis of studying the relative expression of the six genes in different organs, possible physiological functions of these enzymes during the fruit development, seed germination, leaf development and senescence were suggested. 在初步研究6个基因在黄瓜各器官中相对表达量的基础上,探索了这些酶在黄瓜果实发育、种子萌发和叶片发育与衰老等生理过程的作用。
CIRP mRNA relative expression quantity was detected by the double standard curve in the real time PCR method. First, two pairs of specific primers were designed on the nucleic acid sequences of Housekeeping gene GAPDH and rats CIRP, which have been published in the GenBank. 荧光定量PCR方法采用双标准曲线法对CIRPmRNA进行相对定量检测,先从GenBank上公布大鼠的CIRP和持家基因GAPDH的核酸序列设计两对特异性引物并合成。
Compared to IR group, relative expression of HO-1 mRNA in Znpp+ IR group reduced and was still higher than that in control group. Znpp+X线组的HO-1mRNA的相对表达量比X线照射组明显减少,但仍高于空白对照组。
Then use Real-time PCR to detect VEGF expression, taking GAPDH as internal reference, make analysis of relative expression quantity. 然后用Real-timePcR检测VEGF基因表达,进行相对表达量分析。
Intraperitoneal injection of LPS to copy ALI mouse models, RT-PCR was used to detect the relative expression of the different time points of CGRP mRNA in mouse lung. 2. 方法:1.动物模型复制:腹腔注射LPS复制小鼠ALI动物模型,采用RT-PCR法分别检测不同时间点小鼠肺内CGRPmRNA的相对表达量。
The grade of acute rejection was estimated lungs by HE staining of histological sections. The expression of HO-1 protein in lung tissue were measured by immunohistochemistry technique. Western blot was used to assess the relative expression of HO-1 protein quantitatively. HE染色观察移植肺的排斥反应程度和级别;运用免疫组化技术定性判断HO-1蛋白在移植肺中的表达情况;western-blot定量检测HO-1蛋白在移植肺组织中的表达。
The results are summarized as follows. ( 1) The study was conducted to isolate and clone full-length cDNA sequence of GLO from Haliotis discus hannai Ino., and to investigate the relative expression level of GLO mRNA in different tissue and different development phase. 研究结果总结如下:(1)皱纹盘鲍GLO基因的克隆和组织及不同发育阶段的表达。